WebJul 17, 1997 · f, CHO-HERG cells stably expressing wild-type HERG were fractionated as described and detected with antisera against HERG. Unlike actin, HERG localized … WebThis report evaluates the CHO-hERG DUO cell line performance on QPatch when the multi-hole technology was used. It has a specific focus on success rates, stability, pharmacology and bio-physical properties of the assay but it also gives an introduction to some of the methodology used when running multi-holes.

In Vitro Methods for Cardiotoxicity Testing SpringerLink

WebCells. Cultured CHO cells expressing hERG potassium channels were used. The cells ware incubated either at 37 °C, or initially at 37 °C and subsequently ( at 70-80 % confluence) … WebCHO-hERG cells in the exponential growth phase were collected and resuspended in ECS for later use. 3. Patch clamp test. During the test, hERG current was recorded using a conventional whole-cell patch clamp current technique at room temperature. The cells were seeded in a recording chamber on an inverted microscope, and a single cell in the ... homes for sale in the lakes murrells inlet sc

CHO-hERG Cell Line - Aurora Biomed

WebInterestingly, the I57T variant has been reported to produce a loss of function of HERG or Kv7.1 channels, thereby predisposing to long QT syndrome,6,8 indicating that the same KCNE2 variant could cause two different cardiac rhythm disorders, similar to long QT syndrome and Brugada syndrome caused by SCN5A mutations.31,32 Heart Rhythm. WebOur CHO- hERG cell line was developed and validated as a tool for screening compounds which may lead to QT prolongation. Our CHO-hERG cell line: •Stably expresses hERG … homes for sale in the manor milton ga