Cst atf4 antibody

Author: jgib

August undefined, 2024

WebMar 1, 2024 · The SLC7A11 antibody is validated for use in MEFs in Figure 6—figure supplement 1A, corresponding immunoblots quantified in Figure 6—figure supplement 1B, C, F. ... ATF4 rabbit monoclonal: Cell … WebOverview Product name Anti-ATF-4 antibody See all ATF-4 primary antibodies Description Rabbit polyclonal to ATF-4 Host species Rabbit …

ATF-4 (D4B8) Rabbit mAb from Cell Signaling …

WebDec 17, 2024 · BackgroundThe role of activating transcription factor 4 (ATF4) underlying gastric cancer (GC) remains unclear. The purpose of this study was to investigate the expression levels and biological functions of ATF4 in GC.MethodsExpression of ATF4 was detected by quantitative PCR (qPCR), Western blotting, and immunohistochemistry. Cox … WebResearch studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the … the power of the doctor streaming vf

Endoplasmic reticulum stress inhibits AR expression via the

WebPurified anti-ATF4 Antibody. Total cell lysates (15 µg protein) from non-treated and Tunicamycin (Tm) treated (2 µg/mL, six hours) HeLa and 293T were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-ATF4 (clone W16016A) antibody or competitor's antibody used at the ... WebThis antibody has been shown to work in applications such as: Precipitation, Sequencing, ChIP-Seq, ChIP, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot, and Other. ATF-4 (D4B8) Rabbit … WebAug 13, 2024 · This was especially relevant as we found in the public CHIP-seq data base (chip-atlas.org), that the Nrf2 antibody bound to the ATF4 promoter (~1 kB from the TSS) in the cardiovascular HAEC cell ... siethoff gruppe

Hepatic mTORC1 signaling activates ATF4 as part of its …

ATF4 antibody (10835-1-AP) Proteintech - ptglab

Webab31390 has been referenced in 9 publications. Liu Y Hydrogen peroxide induces nucleus pulposus cell apoptosis by ATF4/CHOP signaling pathway. Exp Ther Med 20:3244-3252 (2024). PubMed: 32855694; Liu P et al. … WebNov 1, 2024 · Membranes were then incubated at 4 °C overnight with ATF4 antibody (BioLegend) at 1:1000. After washing with 1x TBST, anti-rat HRP secondary antibody (CST) was used at 1:2000. A list of commercially available antibodies tested in livers and cell lines is included in Supplementary Table 1. 2.6. Gene expression analysis. sietho hotelWebImmunoprecipitation of ATF-4 from extracts of 293 cells, treated with tunicamycin (2 μg/ml, 8 hr), using Rabbit (DA1E) mAb IgG XP ® Isotype … the power of the doctor part 2

"WebOct 22, 2010 · ATF4 is a transcriptional activator of the integrated stress response, a program of gene expression involved in metabolism, nutrient uptake, anti-oxidation, and the activation of additional transcription factors, such as CHOP/GADD153, that can induce apoptosis. ... (Cell Signaling Technologies). ATF4 antibody was prepared against … " - Cst atf4 antibody

Cst atf4 antibody

ATF4 selectively regulates heat nociception and contributes to kines…

WebOct 20, 2024 · ChIP assays with NFATc1 or ATF4 antibody demonstrated that isoproterenol increased NFATc1 and ATF4 binding to their cognate binding elements in RANKL ... MA, USA). CREB1 and phospho-CREB1 antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA), and phospho-ATF4 antibody was … WebThe antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these …

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Webrepresentative of CST, are rejected and are of no force or effect. P roduc t s are labeled wit h For Res earc h Us e O nly or a s imilar labeling s t at ement and hav e not been approv ed, c leared, or lic ens ed by t he FDA or ot her regulat ory f oreign or domes t … WebMar 25, 2024 · Western Blot analysis of Rat Brain showing detection of ~39 kDa (isoform 2) ATF4 protein using Mouse Anti-ATF4 Monoclonal Antibody, Clone S360A-24 (56583). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody (56583) at …

WebOur ATF4 polyclonal, monoclonal and recombinant monoclonal antibodies are developed in Rabbit, Mouse and Goat. These antibodies have been verified by Cell treatment, … WebOct 22, 2010 · ATF4 is a transcriptional activator of the integrated stress response, a program of gene expression involved in metabolism, nutrient uptake, anti-oxidation, and …

WebJun 24, 2024 · PERK IPs were performed with 1 mg of lysate incubated with 2 µg of primary antibody (Proteintech, #20582-1AP) or the corresponding IgG (Cell Signaling Technology, #2729) overnight at 4 °C with ... WebActivating transcription factor 4 (ATF4), also known as cAMP-response element-binding protein 2 (CREB2), is a substrate of RSK2 and a basic leucine-zipper transcription factor …

WebMay 5, 2024 · About 500 mg protein of total cell lysates was incubated with primary antibody anti-ATF4 (#11815, CST or 10835-1-ap, Proteintech group, USA) or anti-CTCF (#3418, CST or sc271514, Santa Cruz Biotechnology, USA) at 4 °C with overnight shaking. The immune complexes were then incubated with Protein A/G Dynabeads (Thermo …

Web(11815) ATF-4 (D4B8) Rabbit mAb - Cell Signaling Technology - CiteAb. 670 citations – A rabbit monoclonal antibody, raised against Cyclic AMP-dependent transcription factor ATF-4 (Human), supplied by Cell Signaling Technology. ... ATF4, Activating transcription factor 4, CREB-2, CREB2, Cyclic AMP-dependent transcription factor ATF-4, Cyclic ... siethotdealsWebATF4 Antibody - Mouse monoclonal antibody to human Activating Transcription Factor 4, clone S360A-24. Validated for WB, IHC, ICC/IF with human, rat tissue. ... Cell Signaling, Epigenetics and Nuclear Signaling, Neuroscience : Cellular Localization : Cell membrane, Cytoplasm, Nucleus : Accession Number : NP_001666.2 ... the power of the doctor piratedWebDec 25, 2024 · The membrane was blocked and then incubated overnight at 4°C with primary antibody as described previously ... antibody to phospho-T389 p70S6K1 was obtained from Cell Signaling Technology (catalog number: 9205); antibodies to REDD1 (catalog number: 10638-1-AP), Sestrin1 (catalog number: 21668-1-AP), and Sestrin2 … siethospWeb1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATF4 (60035-1-Ig, Clone:3E4C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at dilution 1:1000 (red), or 0.4 ug Control … the power of the doctor bbc americaWebMar 3, 2024 · Effective treatments for patients suffering from heat hypersensitivity are lacking, mostly due to our limited understanding of the pathogenic mechanisms underlying this disorder. In the nervous system, activating transcription factor 4 (ATF4) is involved in the regulation of synaptic plasticity and … the power of the doctor watch freeWebMar 21, 2024 · CHST4 (Carbohydrate Sulfotransferase 4) is a Protein Coding gene. Diseases associated with CHST4 include Mucinous Adenocarcinoma and Colon … sie thu myint acta scienceWebAnti-ATF4 antibodies are offered by a number of suppliers. This target gene encodes the protein 'activating transcription factor 4' in humans and may also be known as CREB-2, … siethr