Webwww.ncbi.nlm.nih.gov WebApr 18, 2014 · We show that CTD110.6, #3, and #10 antibodies can be used to detect cell surface glycoproteins bearing O-GlcNAc. Cell surface glycoproteins recognized by …
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WebBinding by antibodies CTD110.6, #3, and #10 was inhibited by 5 m m GlcNAc, but not 5 m m GalNAc, and was thus specific for recognition of O-GlcNAc. However, binding of antibodies 10D8, RL2, and #14 was not inhibited by the presence of 5 m m GlcNAc. Therefore, only antibodies CTD110.6, #3, and #10 should be used for cell surface … WebFor O-glcNAcylation detection, CTD110.6 antibody was covalently conjugated to (4) Defective STIM-mediated store operated Ca2+ entry in hepatocytes leads to metabolic dysfunction in obesity eLife December 15, 2024 Ana Paula Arruda, Benedicte Mengel Pers, Günes Parlakgul, Ekin Güney, Ted Goh, ...
WebSimilar results were obtained for wild-type HEK293T cells (not shown). (C) Expression and O-GlcNAcylation of Notch1, Notch1Δ2xO-GlcNAc and Notch1Δ4xO-GlcNAc were analyzed by immunoprecipitation (IP) using NOTCH1 (8G10) antibody, followed by immunoblotting with CTD110.6 O-GlcNAc or NOTCH1 antibodies. WebJan 1, 2015 · The specificity of the CTD110.6 antibody immunoreactivity was confirmed by a hapten inhibition test using 0.1 m GlcNAc. B, the levels of O-GlcNAc modification on N1EGF1–36-MycHis quantitated by densitometric analyses of immunoblots represented in A (n = 3; results are shown as mean ± S.D.; *, p < 0.01; **, p < 0.05; unpaired Student's t …
WebThus, with careful controls, antibodies CTD110.6 (IgM), #3 (IgG), and #10 (IgG) can be used to detect membrane and secreted proteins modified by O-GlcNAc on EGF repeats. Secreted and cell surface glycoproteins carry a wide spec-trum of N-and O-glycans that are synthesized during transit WebApr 19, 2011 · The O-GlcNAc specific antibody, RL-2, is not N-GlcNAc 2-modifed proteins , so RL-2 antibodies may be more specific for O-GlcNAc-modifed proteins than …
WebThe antibody CTD110.6 is often used to detect changes in the O-GlcNAc modification. Recently, it has been demonstrated that CTD110.6 recognizes N-linked N,N'-diacetylchitobiose, which is thought ...
WebTime course studies by western blot with the CTD110.6 antibody demonstrated that i.v. injection in rat at 50 mg/kg increased global protein O-GlcNAcylation in total brain homogenates, starting at 1 h and peaking at 10 h and decreased pS396 tau/Tau-5 starting at 1 h, peaking at 4 h, and restoring to normal at 16 h (Yuzwa et al., 2008). theragun elite stopped workingWebOct 11, 2013 · For immunodetection, CTD110.6 antibody was used because it is widely used for the detection of O-GlcNAcylated proteins. Compared to the α-DG-Fc isolated from the control transfectants, immunoreactivity of α-DG-Fc towards CTD110.6 was elevated upon GTDC2 overexpression (Fig. 1A). signs and symptoms of a pending heart attackWebCTD110.6, 18B10 and 9D1 are anti-O-GlcNAc monoclonal antibodies. B. HEK 293T cells were treated with DMSO, 50 μM TG or 50 μM 5SG for 24 hours, 200 nM rapamycin … theragun elite - black percussive therapyWebDec 11, 2009 · The following antibodies were used: anti-O-GlcNAc (CTD110.6, Covance), anti-OGT (DM17, Sigma), anti-actin, anti-glycogen phosphorylase ... Korea) for providing the glutamine:fructose-6-phosphate amidotransferase antibody. Supplementary Material. Download : Download Acrobat PDF file (541KB) Recommended articles. REFERENCES … theragun elite black fridayWebResults: Three antibodies recognized cell surface O-GlcNAc and also bound terminal β-GlcNAc on N-glycans. Conclusion: With careful controls, cell surface O-GlcNAc can be detected using antibodies CTD110.6, 18B10.C7, and 9D1.E4. Significance: Antibodies to monitor cell surface O-GlcNAcylated proteins were characterized. theragun cyber monday dealWebO-GlcNAc Antibody (CTD110.6) is an IgM κ mouse monoclonal O-GlcNAc antibody (also designated O-linked β-N-acetylglucosamine antibody, O-β-GlcNAc antibody, O-linked … signs and symptoms of an unwell babyWebPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. signs and symptoms of a peptic ulcer