Hieff unicon® hotstart j-taq dna polymerase
WebDescription. Hieff™ Double-Block Anti-Taq DNA Polymerase Antibody is a double blocking Taq DNA Polymerase monoclonal antibody for hot start PCR. It can inhibit the activity of 5 '- 3' polymerase and 5 '- 3' exonuclease after binding to Taq DNA Polymerase, which can effectively inhibit the nonspecific annealing of primers and the nonspecific ... WebPfuTurbo hotstart DNA polymerase (2.5 U/ l) 100 U 500 U 1000 U 10× Cloned Pfu DNA polymerase reaction buffera 1 ml 2 × 1 ml 4 × 1 ml a See Preparation of Media and Reagents. STORAGE CONDITIONS All components: –20°C ...
Hieff unicon® hotstart j-taq dna polymerase
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Web2×Hieff® PCR Master Mix是即用型的常规PCR预混合溶液,含有Hieff® Taq DNA Polymerase(货号:10101)、dNTP混合物、MgCl2以及优化的缓冲体系。反应时只需加入引物和模板即可进行扩增,大大简化实验的操作步骤。 产品中含有溴酚蓝染料,PCR产物可以直接进行电泳。 WebThis aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. Taq DNA polymerase possesses a 5´→3´ polymerase activity 1,2,3 and a 5´ flap endonuclease activity 4,5. It is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing ...
Web2×Hieff® PCR Master Mix是即用型的常规PCR预混合溶液,含有Hieff® Taq DNA Polymerase(货号:10101)、dNTP混合物、MgCl2以及优化的缓冲体系。反应时只需加入引物和模板即可进行扩增,大大简化实验的操作步骤。 产品中含有溴酚蓝染料,PCR产物可以直接进行电泳。 WebHotStarTaq DNA Polymerase 0.5 µl 2.5 units/reaction Distilled water . Variable – Template DNA (added at step 4) Variable ≤1 µg/reaction Total reaction volume : 100 µl ‡ * Contains 15 mM MgCl 2. † For templates with GC-rich regions or complex secondary structure.
Web6 de ago. de 2015 · “hotstart” Taq ADN polymerase ñượ c t ạ o thành t ừ vi ệ c t r ộ n v ớ i kháng th ể ñơ n dòng anti-Taq ở m ứ c 50, 100 ho ặ c 150 ng kháng th ể v ớ i m ỗ i WebProduct Description. Hieff UNICON ™ Hotstart J-Taq DNA polymerase is a thermostable Taq DNA Polymerase mutant complexed with a proprietary antibody that inhibits polymerase activity below 60℃. The polymerase activity can only be released after heating at 95℃, thus avoiding nonspecific amplification and primer-dimer formation during …
WebGeneral description. JumpStart ™ REDTaq ® DNA Polymerase is Sigma′s high performance Taq DNA Polymerase blended with JumpStart Taq antibody and an inert red dye tracer. Extensive testing with a variety of primers and templates indicates that the performance of JumpStart REDTaq DNA Polymerase is equivalent to, or better than, …
WebPolymerase: Taq DNA polymerase: ... (10717ES) Hieff Unicon™ Hotstart Direct Taq DNA Polymerase, 5 U/μL (10125ES) dNTP Mix (25 mM each) (10726ES) Hieff UNICON™ Hotstart E-Taq DNA Polymerase, 5 U/μL. Related articles >> African Swine Fever Virus - Total Master Mix/Direct Amplification qPCR Solution raystown lake current water temperatureWebHieff Unicon® Hotstart Direct Taq DNA Polymerase是一种耐受血液及其它抑制物的热启动DNA聚合酶。本产品采用抗体封闭,具备良好的扩增灵敏度和特异性。本品在预变性温度下加热30 sec封闭抗体即可完全失活,释放出 DNA 聚合酶活性。使用该热启动Taq酶可以有效抑制引物非特异性退火导致的扩增。 raystown lake dinner cruiseWebHieff UNICON™ Hotstart E-Taq DNA Polymerase is a hot start DNA polymerase with double blocking by double ... Quantity: 1000 U, 500 U, 250 U, 10000 U, 25000 U, 100000 U; Applications: Inquire; Supplier Page. Compare Product. Select All. Select up ... simply giveWebWe offer different hot-start DNA polymerases to support your everyday research needs. This includes Thermo Scientific DreamTaq Hot Start DNA Polymerase, which is an enhanced hot start Taq DNA polymerase suitable for most PCR applications. DreamTaq Hot Start offers higher sensitivity, specificity, and yields compared to conventional hot start ... raystown lake depthWebGostaríamos de lhe mostrar uma descrição aqui, mas o site que está a visitar não nos permite. simply give campaignWebHieff UNICON® Hotstart E-Taq DNA Polymerase is a hot start DNA polymerase with double blocking by double antibodies independently developed by the company. This product not only blocks the 5'→3' polymerase activity of Taq DNA polymerase, but also blocks the 5'→3' exonuclease activity. simplygiving/serviceone barnardosWeb7.1 Phire Hot Start II DNA Polymerase (F-122) Thermostable Phire DNA Polymerase is isolated and purified from an E. coli strain carrying a plasmid with the cloned archaeal DNA polymerase gene. Phire Hot Start II DNA Polymerase possesses the following activities: 5´→3´ DNA polymerase activity and a weak 3´→5´ exonuclease activity. simplygiving.com