Ion chef manual
WebThe Ion Chef™System provides automated, high-throughput template preparation and chip loading for use with the Ion S5™or Ion S5™XL Sequencer. The system includes a complete set of cartridge-based consumables and reagents that enable a user to load two Ion 520™Chips in approximately 13 hours with less than 15 minutes of hands-on time. WebWe have 1 Thermo Scientific Ion Chef manual available for free PDF download: User Manual Thermo Scientific Ion Chef User Manual (78 pages) Brand: Thermo Scientific …
Ion chef manual
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WebThe Ion 540™ Kit – Chef (Cat. no. A27759) contains 10 boxes total: • Ion S5™ Chef Supplies (Part no. A27755; 4 boxes per kit) • Ion S5™ Chef Solutions (Part no. A27754) … WebSee the Ion AmpliSeq™ Library Preparation on the Ion ™ Chef System User Guide (Pub. No. MAN0013432) for more information. The libraries are at ~100 pM (total combined library concentration) and are ready to use in template preparation. Store unused portions of ™ combined libraries at 4°C to 8°C for up to 1 month.
WebIon ChefTM (Life Technologies) to prepare pooled Ion AmpliSeqTM libraries for emulsion PCR and loading sequencing chips. The workload and the number of manual pipetting …
WebThermo Fisher Scientific - US Webneeded. In the Kits tab, select Ion Chef, select the Ion 520™ & Ion 530™ Kit – Chef from the Template Kit dropdown list, select Ion S5™ Sequencing Kit from the Sequencing Kit dropdown list, then enter or scan the barcodes of the Ion Chef™ Library Sample Tubes into the appropriate Sample Tube Label fields of the Plan tab. 4.
WebStart the Ion Chef™ run 1. On the Ion Chef™ home touchscreen, touch Set up run. 2. Touch Step by step, then touch AmpliSeq on the Run Options screen. Note: To bypass the step by step deck loading guide, touch Quick start. 3. Ensure that you have completely loaded the Ion Chef™ deck with Ion AmpliSeq™ Kit for Chef DL8 consumables by ...
WebThe Ion Chef System fully automates library preparation, template preparation, and produces sequencing-ready Ion Torrent semiconductor chips. Step 1 Automated library … truffaut nimes facebookWeb1 dec. 2015 · Boxplot of the loading efficiencies using manual loading and loading with the Ion Chef™. The number of pipetting steps in the manual template preparation … truffaut onlineWebThe Ion Chef™ System must be connected to the Torrent Server either directly using a category 6 Ethernet cable, or indirectly through a local area network that has been … philip heronWebFor Research Use Only. Not for use in diagnostic procedures. Ion Torrent™ Ion CarrierSeq™ ECS Kits USER GUIDE For manual library preparation for use with: IonCode™ Barcode Adapters Ion 530™ Chip Ion 540™ Chip Ion GeneStudio™ S5 Systems Ion S5™ Systems Ion Chef™ System Catalog Numbers A43585, A48022, … truffaut or hitchcock crosswordWebStart the Ion Chef ™ run. Perform the following steps to initiate an Ion AmpliSeq ™ Instrument. run on the Ion Chef ™ 1. On the Ion Chef ™ home touchscreen, touch Set up run. 2. Touch Step by step, then touch AmpliSeq on the Run Options screen. Note: To bypass the step by step deck loading guide, touch Quick start. 3. Confirm that you ... philip hernandez actorWebIon Chef™ System and Torrent Server connectivity • An Ion Chef™ Instrument now supports only one Torrent Server connection. Confirm that your primary Torrent Server connection … philip herringWebIt provides scalable template preparation for all Ion semiconductor chips. With its small footprint, the system fits easily on any bench top. For a fully automated workflow, please see the Ion Chef Instrument. Efficiency and flexibility The Ion OneTouch 2 System integrates multiple manual template preparation steps into a single system. philip hershberg shannon