WebIf you are located in North America, please contact Ion Torrent at: 1-87-SEQUENCE (1-877-378-3623) If you are located outside of North America, please contact Ion Torrent at: +1-203-458-8552 ... The fusion PCR method uses fusion primers to attach the Ion A and truncated P1 (trP1) Adapters to the amplicons as they are generated in PCR. WebIon Torrent™ Ion Xpress™ Barcode Adapters 65 - 80 Kit The Ion Xpress™ Barcode Adapters 65 - 80 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent …
(PDF) Deep sequencing of hepatitis B virus using Ion Torrent …
WebThe Ion Xpress™ Barcode Adapters 17-32 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables ... WebKits contain Adapter P1 in combination with either the non-barcoded Adapter A (KK8330), or a set of eight barcoded A adapters (KK8331– KK8342). Libraries prepared using … shstrdupw+0x290
Deep sequencing of hepatitis B virus using Ion Torrent fusion primer ...
Web12 mrt. 2012 · The version with P1 seems more recent, so probably correct. However, I guess the B and key will be added in the e-PCR. That will only make the 3' end adapter … Web8 feb. 2016 · The first step in the workflow is to generate a library of DNA fragments flanked by the Ion Torrent adapters. This can be done by ligating the adapters to the PCR products or by adding the adapter sequences during PCR by designing PCR primers with the Ion adapter sequences at the 5 end (Fig 4). Web5 mrt. 2024 · Afterwards, we ligated each sample with Ion P1 Adapter and Ion Express barcode (Thermo Fisher Scientific). The ligation was as follows: 15 minutes at 25 °C, 5 minutes at 72 °C. theory tv